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Title: | IMMUNOEPIDEMIOLOGY OF PVRII, A PUTATIVE VACCINE CANDIDATE REPRESENTING PLASMODIUM VIVAX DUFFY BINDING PROTEIN, IN SRI LANKAA |
Authors: | Dias, Sajani Handunnetti, Shiroma M Yazdan, Syed S Chitnis, Chetan E Randenrya, Preethi V |
Issue Date: | 207 |
Publisher: | Lanka.56 th of the Ann. Meeting of the American Society of Tropical Medicine and Hygiene, Philadelphia, USA. Supplement to The American Journal of Tropical Medicine and Hygiene 77 (5), Abstract book -pp 55-56 |
Abstract: | Study of naturally acquired antibody responses to a potential vaccrne candidate ts rmperatrve to provide insights to vaccine deve opment. Recombinant protein PvRll, a putative vaccine candrdate representrng regron ll of native Plasmodrum vivaxDlffy Bindrng Protein (DBP), was used in ELISA to examine the total (lgG+tgM), igM and tgG isotype antibody responses A reductlon sensiiive ELISA was preformed for toial antrbocjies. Se:-a were collected from acute vivax malaria patients from two endemic areas (EAs) ,rvhere low and unstable malaria conditions prevail, and fronr a non-endemic area (NEA) in Sri Lanka. Pre,yalence of totai antibodies was 50% from Colombo (NEA; N=111),46% from Anuradhapura (EA; N=94) and 41ok fron Kataragarna (EA, N=106) Significantly higher prevaience (Chi square, P<0 05) and magnitude (ANOVA, P<0 0't ) of tota antibodies were recorded from NEA compared with E,As. Total antibody parameters rn all test populatrons,,vere independent of aEe of indivrduals, parasite density and previous exposure. Conformation sensitrve anti-pvRll nronoclonai antibody 2H 1 0 reacted only w th non-reduced pvRil but not with its reduced form. Test sera partially reacted with i'educed PvR1l, and this indicating the recognition of irnear B cell eprtopes. A parailr.l increase in total anlibody response to PvRll linear epitopes wrth rncreasrng e\pcsure v.ras detected n residents of Kataragama. No signrficant difference was detected arnong test populatrons either in anti-PvRli lgM prelavence (Cht square. P>0.05) or magnitude (ANOVA, P>0.05). Prevalence of 19G3 (Chi |
URI: | http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5565 |
Appears in Collections: | Department of Zoology |
Files in This Item:
File | Description | Size | Format | |
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12 Premarathne PH 56th MoATMnH USA 2007.pdf | Abstract | 1.35 MB | Adobe PDF | View/Open |
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