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DC Field | Value | Language |
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dc.contributor.author | Herath, H. M. L. P. B | |
dc.contributor.author | Elvitigala, D. A. S | |
dc.contributor.author | Godahewa, G.L | |
dc.contributor.author | Umasuthan, N | |
dc.contributor.author | Whang, I. | |
dc.contributor.author | Noh, J.K. | |
dc.contributor.author | Lee, J | |
dc.date.accessioned | 2021-06-06T08:54:40Z | |
dc.date.available | 2021-06-06T08:54:40Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Herath, H. M. L. P. B., Elvitigala, D. A. S., Godahewa, G. I., Umasuthan, N., Whang, I., Noh, J. K., & Lee, J. (2016). Molecular characterization and comparative expression analysis of two teleostean pro-inflammatory cytokines, IL-1β and IL-8, from Sebastes schlegeli. Gene, 575(2), 732-742. | en_US |
dc.identifier.uri | http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5308 | - |
dc.description.abstract | Interleukin 1β (IL-1β) and interleukin 8 (IL-8) are two major pro-inflammatory cytokines which play a central role in initiation of inflammatory responses against bacterial- and viral-infections. IL-1β is a member of the interleukin 1 family proteins and IL-8 is classified as a CXC-chemokine. In the current study, putative IL-1β and IL-8 counterparts were identified from a black rockfish transcriptomic database and designated as RfIL-1β and RfIL-8. The RfIL-1β cDNA sequence consists of 1140 nucleotides with a 759 bp open reading frame (ORF) which encodes a 252 amino acid (aa) protein, whereas the RfIL-8 cDNA sequence (898 bp) harbors a 300 bp ORF encoding a 99 aa protein. Furthermore, the RfIL-1β aa sequence contains an IL-1 super family-like domain and an N-terminal IL-1 super family propeptide, while the amino acid sequence of RfIL-8 consists of a typical chemokine-CXC domain. Analysis of sequenced BAC clones containing RfIL-1β and RfIL-8 showed each gene to contain 4 exons interrupted by 3 introns. Pairwise comparison and phylogeny analysis of these cytokine sequences clearly revealed their closer relationship with other corresponding members of teleosts compared to birds and mammals. Constitutive differences in RfIL-1β and RfIL-8 mRNA expression were detected in a tissuespecific manner with the highest expression of each mRNA in spleen tissue. Two immune challenge experiments were conducted with Streptococcus iniae and polyinosinic:polycytidylic acid (poly I:C; a viral double stranded RNA mimic), and transcripts were quantified in spleen and peripheral blood cells. Significantly increased RfIL-1β and RfIL8 transcript levels were detected with almost similar profile patterns, further suggesting a putative involvement of these pro-inflammatory cytokines in the rockfish immunity | |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.subject | Pro-inflammatory cytokines | en_US |
dc.subject | Immune responses | en_US |
dc.subject | Genomic arrangement | en_US |
dc.subject | Black rockfish | en_US |
dc.title | Molecular characterization and comparative expression analysis of two teleostean pro-inflammatory cytokines, IL - 1β and IL-8, from sebastes schlegeli | en_US |
dc.type | Article | en_US |
Appears in Collections: | Department of Basic Sciences & Social Sciences |
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