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Title: | Molecular Characterization of a bactericidal permeability - increasing protein / ilpopolysaccharide - binding protein from black rockfish (sebastes schlegelii) : Deciphering its putative antibacterial role |
Authors: | Lee, S. Elvitigala, D. A. S Lee, S. Kim, H. C Park, H. C Lee, J. |
Keywords: | LBP/BPI Black rockfish Antibacterial activity Immune stimulation Transcriptional regulation |
Issue Date: | 2017 |
Publisher: | Elsevier |
Citation: | Lee, S., Elvitigala, D. A. S., Lee, S., Kim, H. C., Park, H. C., & Lee, J. (2017). Molecular characterization of a bactericidal permeability-increasing protein/lipopolysaccharide-binding protein from black rockfish (Sebastes schlegelii): Deciphering its putative antibacterial role. Developmental & Comparative Immunology, 67, 266-275. |
Abstract: | Bactericidal permeability-increasing protein (BPI)/lipopolysaccharide (LPS) binding proteins (LBPs) are well-known proteins that play an indispensable role in host antimicrobial defense. Herein, we report a homolog of BPI/LBP from black rockfish (Sebastes schlegelii) (designated as RfBPI/LBP) and characterize its structural and functional features at the molecular level. We identified the putative complete open reading frame (1422 bp) of RfLBP that encodes a 474 amino acid protein with a predicted molecular mass of ~51.5 kDa. The primary protein sequence of RfBPI/LBP contains domain features of BPI/LBP family proteins and shares significant sequence consistency with its homologs. Our phylogenetic analysis clearly demonstrated the vertebrate ancestral origin of RfBPI/LBP, further reinforcing its evolutionary relationship with teleostean homologs. Recombinant RfBPI/LBP demonstrated in vitro LPS-binding activity and antibacterial activity against Escherichia coli, but not against Streptococcus iniae. Moreover, RfBPI/LBP exhibited temporal transcriptional activation against pathogens and pathogen-associated molecular patterns. Collectively, our findings suggest that RfBPI/LBP plays an essential role in host antimicrobial defense, plausibly through selective eradication of invading bacteria |
URI: | http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5306 |
Appears in Collections: | Department of Basic Sciences & Social Sciences |
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