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dc.contributor.authorFernando, I. P. Shanura-
dc.contributor.authorSanjeewa, Kalu Kapuge Asanka-
dc.contributor.authorLee, H. G.-
dc.contributor.authorKim, Hyun-Soo-
dc.contributor.authorVaas, A. P J. Prasanna-
dc.contributor.authorDe Silva, H Ireshika Chathurani-
dc.contributor.authorNanayakkara, C.M.-
dc.contributor.authorAbeytunga, D Thusitha Udayangani-
dc.contributor.authorLee, Dae-Sung-
dc.contributor.authorLee, Jung-Suck-
dc.contributor.authorJeon, You-Jin-
dc.date.accessioned2021-06-05T10:30:18Z-
dc.date.available2021-06-05T10:30:18Z-
dc.date.issued2020-
dc.identifier.citation. P. Shanura Fernando, K. K. Asanka Sanjeewa, H. G. Lee, Hyun-Soo Kim, A. P J. Prasanna Vaas, H Ireshika Chathurani De Silva, Chandrika MalkanthiNanayakkara, D Thusitha Udayangani Abeytunga, Dae-Sung Lee, Jung-Suck Lee, and You-Jin Jeon (2020). Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells, Marine Drugs 18, 196;en_US
dc.identifier.uridoi:10.3390/md18040196-
dc.identifier.urihttp://archive.cmb.ac.lk:8080/xmlui/handle/70130/5270-
dc.description.abstractFucoidans are biocompatible, heterogeneous, and fucose rich sulfated polysaccharides biosynthesized in brown algae, which are renowned for their broad-spectrum biofunctional properties. As a continuation of our preliminary screening studies, the present work was undertaken to extract polysaccharides from the edible brown algae Sargassum polycystum by a modified enzyme assisted extraction process using Celluclast, a food-grade cellulase, and to purify fucoidan by DEAE-cellulose anion exchange chromatography. The apoptotic and antiproliferative properties of the purified fucoidan (F5) were evaluated on HL-60 and MCF-7 cells. Structural features were characterized by FTIR and NMR analysis. F5 indicated profound antiproliferative effects on HL-60 leukemia and MCF-7 breast cancer cells with IC50 values of 84.63 ± 0.08 μg mL−1 and 93.62 ± 3.53 μg mL−1 respectively. Further, F5 treatment increased the apoptotic body formation, DNA damage, and accumulation of HL-60 and MCF-7 cells in the Sub-G1 phase of the cell cycle. The effects were found to proceed via the mitochondria-mediated apoptosis pathway. The Celluclast assisted extraction is a cost-efficient method of yielding fucoidan. With further studies in place, purified fucoidan of S. polycystum could be applied as functional ingredients in food and pharmaceuticals.en_US
dc.language.isoenen_US
dc.subjectSri Lankan algae; anticancer; sulfated polysaccharide; fucoidan; Celluclast; sargassumen_US
dc.titleFucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cellsen_US
dc.typeArticleen_US
Appears in Collections:Department of Plant Sciences

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