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DC Field | Value | Language |
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dc.contributor.author | Fernando, I. P. Shanura | - |
dc.contributor.author | Sanjeewa, Kalu Kapuge Asanka | - |
dc.contributor.author | Lee, H. G. | - |
dc.contributor.author | Kim, Hyun-Soo | - |
dc.contributor.author | Vaas, A. P J. Prasanna | - |
dc.contributor.author | De Silva, H Ireshika Chathurani | - |
dc.contributor.author | Nanayakkara, C.M. | - |
dc.contributor.author | Abeytunga, D Thusitha Udayangani | - |
dc.contributor.author | Lee, Dae-Sung | - |
dc.contributor.author | Lee, Jung-Suck | - |
dc.contributor.author | Jeon, You-Jin | - |
dc.date.accessioned | 2021-06-05T10:30:18Z | - |
dc.date.available | 2021-06-05T10:30:18Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | . P. Shanura Fernando, K. K. Asanka Sanjeewa, H. G. Lee, Hyun-Soo Kim, A. P J. Prasanna Vaas, H Ireshika Chathurani De Silva, Chandrika MalkanthiNanayakkara, D Thusitha Udayangani Abeytunga, Dae-Sung Lee, Jung-Suck Lee, and You-Jin Jeon (2020). Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells, Marine Drugs 18, 196; | en_US |
dc.identifier.uri | doi:10.3390/md18040196 | - |
dc.identifier.uri | http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5270 | - |
dc.description.abstract | Fucoidans are biocompatible, heterogeneous, and fucose rich sulfated polysaccharides biosynthesized in brown algae, which are renowned for their broad-spectrum biofunctional properties. As a continuation of our preliminary screening studies, the present work was undertaken to extract polysaccharides from the edible brown algae Sargassum polycystum by a modified enzyme assisted extraction process using Celluclast, a food-grade cellulase, and to purify fucoidan by DEAE-cellulose anion exchange chromatography. The apoptotic and antiproliferative properties of the purified fucoidan (F5) were evaluated on HL-60 and MCF-7 cells. Structural features were characterized by FTIR and NMR analysis. F5 indicated profound antiproliferative effects on HL-60 leukemia and MCF-7 breast cancer cells with IC50 values of 84.63 ± 0.08 μg mL−1 and 93.62 ± 3.53 μg mL−1 respectively. Further, F5 treatment increased the apoptotic body formation, DNA damage, and accumulation of HL-60 and MCF-7 cells in the Sub-G1 phase of the cell cycle. The effects were found to proceed via the mitochondria-mediated apoptosis pathway. The Celluclast assisted extraction is a cost-efficient method of yielding fucoidan. With further studies in place, purified fucoidan of S. polycystum could be applied as functional ingredients in food and pharmaceuticals. | en_US |
dc.language.iso | en | en_US |
dc.subject | Sri Lankan algae; anticancer; sulfated polysaccharide; fucoidan; Celluclast; sargassum | en_US |
dc.title | Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells | en_US |
dc.type | Article | en_US |
Appears in Collections: | Department of Plant Sciences |
Files in This Item:
File | Description | Size | Format | |
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FernandoMarinedrugs-18-00196.pdf | 2.43 MB | Adobe PDF | View/Open |
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