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Title: | Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells |
Authors: | Fernando, I. P. Shanura Sanjeewa, Kalu Kapuge Asanka Lee, H. G. Kim, Hyun-Soo Vaas, A. P J. Prasanna De Silva, H Ireshika Chathurani Nanayakkara, C.M. Abeytunga, D Thusitha Udayangani Lee, Dae-Sung Lee, Jung-Suck Jeon, You-Jin |
Keywords: | Sri Lankan algae; anticancer; sulfated polysaccharide; fucoidan; Celluclast; sargassum |
Issue Date: | 2020 |
Citation: | . P. Shanura Fernando, K. K. Asanka Sanjeewa, H. G. Lee, Hyun-Soo Kim, A. P J. Prasanna Vaas, H Ireshika Chathurani De Silva, Chandrika MalkanthiNanayakkara, D Thusitha Udayangani Abeytunga, Dae-Sung Lee, Jung-Suck Lee, and You-Jin Jeon (2020). Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells, Marine Drugs 18, 196; |
Abstract: | Fucoidans are biocompatible, heterogeneous, and fucose rich sulfated polysaccharides biosynthesized in brown algae, which are renowned for their broad-spectrum biofunctional properties. As a continuation of our preliminary screening studies, the present work was undertaken to extract polysaccharides from the edible brown algae Sargassum polycystum by a modified enzyme assisted extraction process using Celluclast, a food-grade cellulase, and to purify fucoidan by DEAE-cellulose anion exchange chromatography. The apoptotic and antiproliferative properties of the purified fucoidan (F5) were evaluated on HL-60 and MCF-7 cells. Structural features were characterized by FTIR and NMR analysis. F5 indicated profound antiproliferative effects on HL-60 leukemia and MCF-7 breast cancer cells with IC50 values of 84.63 ± 0.08 μg mL−1 and 93.62 ± 3.53 μg mL−1 respectively. Further, F5 treatment increased the apoptotic body formation, DNA damage, and accumulation of HL-60 and MCF-7 cells in the Sub-G1 phase of the cell cycle. The effects were found to proceed via the mitochondria-mediated apoptosis pathway. The Celluclast assisted extraction is a cost-efficient method of yielding fucoidan. With further studies in place, purified fucoidan of S. polycystum could be applied as functional ingredients in food and pharmaceuticals. |
URI: | doi:10.3390/md18040196 http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5270 |
Appears in Collections: | Department of Plant Sciences |
Files in This Item:
File | Description | Size | Format | |
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FernandoMarinedrugs-18-00196.pdf | 2.43 MB | Adobe PDF | View/Open |
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