Please use this identifier to cite or link to this item: http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5270
Title: Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells
Authors: Fernando, I. P. Shanura
Sanjeewa, Kalu Kapuge Asanka
Lee, H. G.
Kim, Hyun-Soo
Vaas, A. P J. Prasanna
De Silva, H Ireshika Chathurani
Nanayakkara, C.M.
Abeytunga, D Thusitha Udayangani
Lee, Dae-Sung
Lee, Jung-Suck
Jeon, You-Jin
Keywords: Sri Lankan algae; anticancer; sulfated polysaccharide; fucoidan; Celluclast; sargassum
Issue Date: 2020
Citation: . P. Shanura Fernando, K. K. Asanka Sanjeewa, H. G. Lee, Hyun-Soo Kim, A. P J. Prasanna Vaas, H Ireshika Chathurani De Silva, Chandrika MalkanthiNanayakkara, D Thusitha Udayangani Abeytunga, Dae-Sung Lee, Jung-Suck Lee, and You-Jin Jeon (2020). Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells, Marine Drugs 18, 196;
Abstract: Fucoidans are biocompatible, heterogeneous, and fucose rich sulfated polysaccharides biosynthesized in brown algae, which are renowned for their broad-spectrum biofunctional properties. As a continuation of our preliminary screening studies, the present work was undertaken to extract polysaccharides from the edible brown algae Sargassum polycystum by a modified enzyme assisted extraction process using Celluclast, a food-grade cellulase, and to purify fucoidan by DEAE-cellulose anion exchange chromatography. The apoptotic and antiproliferative properties of the purified fucoidan (F5) were evaluated on HL-60 and MCF-7 cells. Structural features were characterized by FTIR and NMR analysis. F5 indicated profound antiproliferative effects on HL-60 leukemia and MCF-7 breast cancer cells with IC50 values of 84.63 ± 0.08 μg mL−1 and 93.62 ± 3.53 μg mL−1 respectively. Further, F5 treatment increased the apoptotic body formation, DNA damage, and accumulation of HL-60 and MCF-7 cells in the Sub-G1 phase of the cell cycle. The effects were found to proceed via the mitochondria-mediated apoptosis pathway. The Celluclast assisted extraction is a cost-efficient method of yielding fucoidan. With further studies in place, purified fucoidan of S. polycystum could be applied as functional ingredients in food and pharmaceuticals.
URI: doi:10.3390/md18040196
http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5270
Appears in Collections:Department of Plant Sciences

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