Please use this identifier to cite or link to this item: http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5260
Full metadata record
DC FieldValueLanguage
dc.contributor.authorAmarasekara, D. S.-
dc.contributor.authorWijerathna, S.-
dc.contributor.authorFernando, C.-
dc.contributor.authorUdagama, P. V.-
dc.date.accessioned2021-06-05T10:27:02Z-
dc.date.available2021-06-05T10:27:02Z-
dc.date.issued2012-
dc.identifier.citation20en_US
dc.identifier.otherhttps://doi.org/10.1111/and.12043-
dc.identifier.urihttp://archive.cmb.ac.lk:8080/xmlui/handle/70130/5260-
dc.description.abstractSeminal oxidative stress plays an important role in male factor infertility (MFI), worldwide. A study was thus undertaken for the first time to establish seminal reactive oxygen species (ROS) as a clinical marker of MFI in a cohort of Sri Lankan males. The nitro blue tetrazolium (NBT) assay for ROS estimation and modified Endtz test for detecting leucocytes were carried out on semen samples (N = 102) of sub-fertile males. Age-matched individuals (N = 30) with proven past paternity served as controls. Significantly higher ROS production was evident in individuals with asthenozoospermia and unexplained infertility (Mann–Whitney U-test, P = 0.000), than of the fertile and the other sub-fertile groups tested. Receiver operating characteristic plot analysis established cut-off points of 40.57 and 42.02 lg formazan/107 spermatozoa for ROS to distinguish fertile males from asthenozoospermics (71.4% sensitivity: 70% specificity; AUC = 0.82), and from unexplained infertile males (74.1 % sensitivity: 73.3% specificity; AUC = 0.85) respectively. As ROS appear to be a potential marker of male infertility, it is imperative to validate this test as a simple, cost-effective hence a widely accessible diagnostic tool to be included in MFI investigations in the developing worlden_US
dc.description.sponsorshipFinancial assistance by the Institute of Biochemistry, Molecular Biology and Biotechnology of the University of Colombo, Sri Lanka is acknowledgeden_US
dc.language.isoenen_US
dc.publisherAndrologiaen_US
dc.subjectAsthenozoospermiaen_US
dc.subjectreactive oxygen speciesen_US
dc.subjectunexplained infertilityen_US
dc.subjectmale factor infertilityen_US
dc.subjectoxidative stressen_US
dc.titleCost-effective diagnosis of male oxidative stress using the nitroblue tetrazolium test: useful application for the developing worlden_US
dc.typeArticleen_US
Appears in Collections:Department of Zoology

Files in This Item:
File Description SizeFormat 
36.pdfMain article277.59 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.