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DC Field | Value | Language |
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dc.contributor.author | Amarasekara, D. S. | - |
dc.contributor.author | Wijerathna, S. | - |
dc.contributor.author | Fernando, C. | - |
dc.contributor.author | Udagama, P. V. | - |
dc.date.accessioned | 2021-06-05T10:27:02Z | - |
dc.date.available | 2021-06-05T10:27:02Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | 20 | en_US |
dc.identifier.other | https://doi.org/10.1111/and.12043 | - |
dc.identifier.uri | http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5260 | - |
dc.description.abstract | Seminal oxidative stress plays an important role in male factor infertility (MFI), worldwide. A study was thus undertaken for the first time to establish seminal reactive oxygen species (ROS) as a clinical marker of MFI in a cohort of Sri Lankan males. The nitro blue tetrazolium (NBT) assay for ROS estimation and modified Endtz test for detecting leucocytes were carried out on semen samples (N = 102) of sub-fertile males. Age-matched individuals (N = 30) with proven past paternity served as controls. Significantly higher ROS production was evident in individuals with asthenozoospermia and unexplained infertility (Mann–Whitney U-test, P = 0.000), than of the fertile and the other sub-fertile groups tested. Receiver operating characteristic plot analysis established cut-off points of 40.57 and 42.02 lg formazan/107 spermatozoa for ROS to distinguish fertile males from asthenozoospermics (71.4% sensitivity: 70% specificity; AUC = 0.82), and from unexplained infertile males (74.1 % sensitivity: 73.3% specificity; AUC = 0.85) respectively. As ROS appear to be a potential marker of male infertility, it is imperative to validate this test as a simple, cost-effective hence a widely accessible diagnostic tool to be included in MFI investigations in the developing world | en_US |
dc.description.sponsorship | Financial assistance by the Institute of Biochemistry, Molecular Biology and Biotechnology of the University of Colombo, Sri Lanka is acknowledged | en_US |
dc.language.iso | en | en_US |
dc.publisher | Andrologia | en_US |
dc.subject | Asthenozoospermia | en_US |
dc.subject | reactive oxygen species | en_US |
dc.subject | unexplained infertility | en_US |
dc.subject | male factor infertility | en_US |
dc.subject | oxidative stress | en_US |
dc.title | Cost-effective diagnosis of male oxidative stress using the nitroblue tetrazolium test: useful application for the developing world | en_US |
dc.type | Article | en_US |
Appears in Collections: | Department of Zoology |
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