Please use this identifier to cite or link to this item: http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5212
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dc.contributor.authorDel Portillo, Hernando A.-
dc.contributor.authorGysin, Jurg-
dc.contributor.authorMattie, Denise M.-
dc.contributor.authorKhouri, Elisabeth-
dc.contributor.authorUdagama, Preethi V.-
dc.contributor.authorMendis, Kamini N.-
dc.contributor.authorDavid, Peter H-
dc.date.accessioned2021-05-23T12:37:27Z-
dc.date.available2021-05-23T12:37:27Z-
dc.date.issued1988-
dc.identifier.citation36en_US
dc.identifier.otherhttps://doi.org/10.1016/0014-4894(88)90081-1-
dc.identifier.urihttp://archive.cmb.ac.lk:8080/xmlui/handle/70130/5212-
dc.descriptionPlasmodium vivax,' Blood-stage surface antigen; DNA sequence; Deoxyribonucleic acid (DNA); Indirect immunofluorescence antibody test (IFAT); Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); Kilobase (kb); Kilo daltons (kDa); Molecular weight range (M); Base pair (bp).en_US
dc.description.abstractPlasmodium vivax is a highly prevalent malaria pathogen of man; the following report is the first to describe the cloning and expression of a major asexual erythrocytic stage antigen of this species. The screening of a genomic DNA expression lilrary with a monoclonal antibody directed against a 200-kDa surface component (Pv200) of the more mature schizonts of P. vivax led to the selection of a recombinant bacterial clone which produced a fusion protein. Mouse and rabbit immune sera raised against the purified fusion protein recognized the 200-kDa parasite antigen on Western blots and reacted with the surface of segmenters by immunofluorescence. Sequencing of the 1.9-kb P. vivax DNA insert coding for this fusion protein revealed a 4547Vo homology at the nucleotide level with the P . falciparum gene of a parasite surface antigen,Pflgs, which has been shown to be a promising candidate for a malaria vaccine in primates and in manen_US
dc.description.sponsorshipSupported by the United Nations Development Program/World Bank/IWorld Health Organization Special Program for Research and Training in Tropical Diseases and by the Institut National de la Sante et de la Recherche Medicale (Contract n" 851009).en_US
dc.language.isoenen_US
dc.publisherExperimental Parasitologyen_US
dc.subjectBlood-stage surface antigenen_US
dc.subjectPlasmodium vivaxen_US
dc.subjectDNA sequenceen_US
dc.titlePlasmadium vivax: Cloning and Expression of a Major Blood-Stage . Surface Antigenen_US
dc.typeArticleen_US
Appears in Collections:Department of Zoology

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