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DC Field | Value | Language |
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dc.contributor.author | Del Portillo, Hernando A. | - |
dc.contributor.author | Gysin, Jurg | - |
dc.contributor.author | Mattie, Denise M. | - |
dc.contributor.author | Khouri, Elisabeth | - |
dc.contributor.author | Udagama, Preethi V. | - |
dc.contributor.author | Mendis, Kamini N. | - |
dc.contributor.author | David, Peter H | - |
dc.date.accessioned | 2021-05-23T12:37:27Z | - |
dc.date.available | 2021-05-23T12:37:27Z | - |
dc.date.issued | 1988 | - |
dc.identifier.citation | 36 | en_US |
dc.identifier.other | https://doi.org/10.1016/0014-4894(88)90081-1 | - |
dc.identifier.uri | http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5212 | - |
dc.description | Plasmodium vivax,' Blood-stage surface antigen; DNA sequence; Deoxyribonucleic acid (DNA); Indirect immunofluorescence antibody test (IFAT); Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); Kilobase (kb); Kilo daltons (kDa); Molecular weight range (M); Base pair (bp). | en_US |
dc.description.abstract | Plasmodium vivax is a highly prevalent malaria pathogen of man; the following report is the first to describe the cloning and expression of a major asexual erythrocytic stage antigen of this species. The screening of a genomic DNA expression lilrary with a monoclonal antibody directed against a 200-kDa surface component (Pv200) of the more mature schizonts of P. vivax led to the selection of a recombinant bacterial clone which produced a fusion protein. Mouse and rabbit immune sera raised against the purified fusion protein recognized the 200-kDa parasite antigen on Western blots and reacted with the surface of segmenters by immunofluorescence. Sequencing of the 1.9-kb P. vivax DNA insert coding for this fusion protein revealed a 4547Vo homology at the nucleotide level with the P . falciparum gene of a parasite surface antigen,Pflgs, which has been shown to be a promising candidate for a malaria vaccine in primates and in man | en_US |
dc.description.sponsorship | Supported by the United Nations Development Program/World Bank/IWorld Health Organization Special Program for Research and Training in Tropical Diseases and by the Institut National de la Sante et de la Recherche Medicale (Contract n" 851009). | en_US |
dc.language.iso | en | en_US |
dc.publisher | Experimental Parasitology | en_US |
dc.subject | Blood-stage surface antigen | en_US |
dc.subject | Plasmodium vivax | en_US |
dc.subject | DNA sequence | en_US |
dc.title | Plasmadium vivax: Cloning and Expression of a Major Blood-Stage . Surface Antigen | en_US |
dc.type | Article | en_US |
Appears in Collections: | Department of Zoology |
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